This mode is a reference to the Hitman series. Press L to activate this mode. It cannot be deactivated until the game is reset or the day ends. Once pressed, Yandere-chan will resemble the main character Agent 47. 47 Mode will make her bald, have a barcode on the back of her head, and change her uniform to a black jacket with a red bow. The song, Ave Maria will play the entire time.
- If Yandere-chan wears gloves during this mode her uniform texture will break.
- This mode was implemented in the June 17th, 2015 Build.
- Press D to disable the copyrighted music.
- The game's warning referenced this mode, saying that this game contains "Bald assassins". However, YandereDev removed it in the March 17th, 2016 Build because he thought it was "cringey".
- The player is able to become bald with the barcode by pressing the H button until they reach the final hairstyle.