Press E to activate this mode. When pressed, Yandere-chan will become Ebola-chan. She will become deathly pale with golden eyes. Her hair is styled in waist-length pink pigtails, with a white flower in it, the tips of her pigtails have a curl that looks similar to the Ebola virus. Yandere-chan will also have small light purple bat wings and her uniform will be recolored to resemble a nurse's outfit. When idle, her arms will be splayed happily outwards.
Any NPC with a complete AI she touches will instantly die (presumably from Ebola) and will exude a visible white vapor that resembles a skull. Senpai and the teachers can be killed in this mode if they touch Ebola-chan.
- If Yandere-chan tries to pick up or drag a corpse, after dropping it, she will no longer have a smile, nor the Ebola-chan animation.
- If Yandere-chan takes off the school uniform, her skin will become regular instead of white, and her eyes will be the default black instead of gold.
- If Yandere-chan kills Senpai and dismembers him with the circular saw, she will no longer be able to run.
- This mode was implemented in the May 4th, 2016 Build.
- If an NPC is killed by Ebola-chan, the NPC will have a yellow outline in Yandere Vision. However, teachers, Senpai, and Midori Gurin will have the regular orange outline.
- YandereDev publicly promised to implement this mode in 2014. It only took an hour for this mode to be implemented.
- Genka Kunahito, Kocho Shuyona, Geiju Tsuka, the Placeholder Club Leaders and Rival-chan cannot be killed with this mode.
- The hair was modeled by Druelbozo.
- YandereDev jokes that since a couple of people were offended by this mode, he should have added Malaria-sama, AIDS-san, Cancer-san, and Bubonic Plague-sama.
- The music is shared with Tornado Mode and Blade Hair Mode.